Plant Genome Editing With Crispr Systems Methods And Protocols 1917 Methods In Molecular Biology, 1917

Yiping Qi

Plant Genome Editing With Crispr Systems Methods And Protocols 1917 Methods In Molecular Biology, 1917
PrefaceTable of ContentsContributing Authors
Part I Review on Plant DNA Repair and Genome Editing1.Plant DNA Repair Pathways and their Applications in Genome EditingQiudeng Que, Zhongying Chen, Tim Kelliher, David Skibbe, Shujie Dong, and Mary-Dell Chilton
Part II CRISPR Design and Mutation Analysis
2.Rapid Screening of CRISPR/Cas9-Induced Mutants using the ACT-PCR MethodChun Wang and Kejian Wang
3.Decoding Sanger...

Plant Genome Editing With Crispr Systems Methods And...

Plant Genome Editing With Crispr Systems Methods And Protocols 1917 Methods In Molecular Biology, 1917

Preface

Table of Contents

Contributing Authors

Part I Review on Plant DNA Repair and Genome Editing

1.Plant DNA Repair Pathways and their Applications in Genome Editing

Qiudeng Que, Zhongying Chen, Tim Kelliher, David Skibbe, Shujie Dong, and Mary-Dell Chilton

Part II CRISPR Design and Mutation Analysis

2.Rapid Screening of CRISPR/Cas9-Induced Mutants using the ACT-PCR Method

Chun Wang and Kejian Wang

3.Decoding Sanger-Sequencing Chromatograms from CRISPR-Induced Mutations

Xianrong Xie, Xingliang Ma, and Yao-Guang Liu

Part III Multiplexed CRISPR-Cas9 Systems

4.Creating Large Chromosomal Deletions in Rice using CRISPR/Cas9

Riqing Li, Si Nian Char, and Bing Yang

5.A Multiplexed CRISPR-Cas9 Editing System Based on the Endogenous tRNA Processing

Kabin Xie and Yinong Yang

6.A Single Transcript CRISPR-Cas9 System for Multiplex Genome Editing in Plants

Xu Tang, Zhaohui Zhong, Qiurong Ren, Binglin Liu, and Yong Zhang

7.CRISPR-Act2.0: An Improved Multiplexed System for Plant Transcriptional Activation

Aimee Malzahn, Yong Zhang, and Yiping Qi

Part IV CRISPR-Cas9 Editing in Monocots

8.Generating Photoperiod-Sensitive Genic Male Sterile Rice Lines with CRISPR-Cas9

Weihang Gu, Dabing Zhang, Yiping Qi, and Zheng Yuan

9.Knocking Out MicroRNA Genes in Rice with CRISPR/Cas9

Jianping Zhou, Zhaohui Zhong, Hongqiao Chen, Mengqiao Yang, Qian Li, Xuelian Zheng, Yiping Qi, and Yong Zhang

10.An Agrobacterium-Mediated CRISPR-Cas9 Platform for Genome Editing in Maize

Keunsub Lee, Huilan Zhu, Bing Yang, and Kan Wang

Part V CRISPR-Cas9 Editing in Dicots

11. Fluorescence Marker-Assisted Isolation of Cas9-Free and CRISPR-Edited Arabidopsis Plants

Hanchuanzhi Yu and Yunde Zhao

12.Creating Targeted Gene Knock-Outs in Brassica oleracea using CRISPR/Cas9

Tom Lawrenson, Penny Hundleby, and Wendy Harwood

13.Application of CRISPR/Cas9-Mediated Gene Editing in Tomato

Nathan T. Reem and Joyce Van Eck

14.Genome Editing in Potato with CRISPR/Cas9

Satya Swathi Nadakuduti, Colby G. Starker, Daniel F. Voytas, C. Robin Buell, and David S. Douches

15.Visual Assay for Gene Editing using a CRISPR/Cas9 System in Carrot Cells

Magdalena Klimek-Chodacka, Tomasz Oleszkiewicz, and Rafal Baranski

16.Genome Editing in Soybean with CRISPR/Cas9

Junqi Liu, Samatha Gunapati, Nicole T. Mihelich, Adrian O. Stec, Jean-Michel Michno, and Robert M. Stupar

17.Genome Editing in Citrus Tree with CRISPR/Cas9

Hongge Jia, Xiuping Zou, Vladimir Orbovic, and Nian Wang

Part VI CRISPR-Cas12a Editing Systems

18.Plant Gene Knockout and Knockdown by CRISPR-Cpf1 (Cas12a) Systems

Yingxiao Zhang, Yong Zhang, and Yiping Qi

19.Editing a Stomatal Developmental Gene in Rice with CRISPR/Cpf1

Abhishek Anand, Xiaojia Yin, Paul Quick, and Anindya Bandyopadhyay

20.Targeted Mutagenesis using FnCpf1 in Tobacco

Akira Endo and Seiichi Toki

Part VII Precise Gene Editing

21.Gene Replacement by Intron Targeting with CRISPR-Cas9

Jun Li, Xiangbing Meng, Jiayang Li, and Caixia Gao

22.Targeted Base Editing with CRISPR-Deamina

Plant Genome Editing With Crispr Systems Methods And Protocols 1917 Methods In Molecular Biology, 1917

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Editora: Humana

Idiomas: Inglês

Data de lançamento: 05/01/2019

Peso: 0,0

Série/Edição Limitada: 1st ed. 2019

EAN: 9781493989904

Plant Genome Editing With Crispr Systems Methods And Protocols 1917 Methods In Molecular Biology, 1917

Yiping Qi

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